Ethanol Leaf Extract of Hoslundia opposita in In Vivo Antioxidant and Hepatoprotective Activity Using an Animal Model

Fred Coolborn Akharaiyi, Edo State University UzairueFollow
Odoligie Imarhiagbe, Edo State University UzairueFollow
Lucky Efe Isunu, Federal University of Technology, AkureFollow
Adebayo Thomas Ajibola, College of Health Science and Technology, Ijero-EkitiFollow

Abstract

Introduction and aim: The liver is a useful organ that is responsible for the detoxification of harmful substances from the body. Its vital role is of importance hence it should be protected from damages in order to function normally. The management of liver diseases with orthodox medicine has been found to have side effects thus its effective management with folklore has been found better.

Materials and Methods: The leaves of H. oppisota harvested from a forest was processed and extracted with ethanol. The extract concentrations of 100 to 400 mg/ml was used to evaluate acute toxicity, biochemical analysis, in vivo antioxidant and histopathology using an animal model.

Aim: The aim of this study was to evaluate the toxicity and antioxidants potentials of H. opposita

Results: 400 mg/kg was found as the LD₅₀ of the plant extract. A decrease in uric acid, cholesterol, urea, creatinine, and bilirubin contents was observed in the single extract-treated mice and the paracetamol-induced but co-administered with extracts, while increased values were observed for protein and albumin contents. The positive control values of ALT, AST, and ALP were 66.74±3.51 IU/L, 68.52±3.63 IU/L, and 342±3.04 IU/L respectively, in the negative control, values were 48.16±3.68 IU/L, 37.46±1.52, and 89.34±2.66 IU/L. A decrease in values was observed for lipid peroxidation in the extract-treated and satellite groups, while, increase values were observed for catalase and glutathione biochemical activities. The overdose of paracetamol, that caused hepatic damages were restored with the ethanol leaf extract over time.

Conclusion: The irregularities in the in vivo biochemical, in vivo antioxidant values and the hepatic damages caused by paracetamol toxicity was regulated on extract treatment. Thereby suggesting its use traditionally for the treatment of liver diseases.

Recommended Citation

Akharaiyi, Fred Coolborn; Imarhiagbe, Odoligie; Isunu, Lucky Efe; and Ajibola, Adebayo Thomas (2022) "Ethanol Leaf Extract of Hoslundia opposita in In Vivo Antioxidant and Hepatoprotective Activity Using an Animal Model," BioMedicine: Vol. 12 : Iss. 3 , Article 7.
DOI: 10.37796/2211-8039.1321