High levels of Histone H3 K27 acetylation and tri-methylation are associated with shorter survival in Oral Squamous Cell Carcinoma patients

Akram Shahhosseini, Department of Clinical Biochemistry, School of Medicine, Shahid beheshti University of Medical Sciences, Tehran, Iran
Ekaterina Bourova-Flin, CNRS UMR 5309/INSERM U1209/University Grenoble-Alpes/Institute for Advanced Biosciences, La Tronche, FranceFollow
Samira Derakhshan, Oral and Maxillofacial Pathology Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Pouyan Aminishakib, Oral and Maxillofacial Pathology Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Afsaneh Goudarzi, Department of Clinical Biochemistry, School of Medicine, Shahid beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background: Acetylation and trimethylation of histone H3 lysine 27 (H3K27ac and H3K27me3) generally activate and repress transcription, respectively. Concurrent activation of H3K27ac and H3K27me3 has been reported to correlate with poor prognosis in hepatocellular carcinoma. A high level of H3K27me3 has been shown to be associated with advanced oral squamous cell carcinoma (OSCC) tumour stage, but prognostic impact of H3K27ac level alone/or in combination with H3K27me3 in OSCC patients had not yet been reported. Material and Methods: In this study, immunohistochemistry using specific antibodies against H3K27ac and H3K27me3 was performed on a series of 72 OSCC samples to investigate the association between H3K27ac and H3K27me3 levels and OSCC patient’s survival. For each mark, the proportion of labelled cells (percentage) and the intensity of the labelling were measured, and a score of percentage x intensity was calculated. Results: A high percentage of H3K27me3 positive cells was significantly associated with survival in univariate and multivariate analyses (logrank p-value

Recommended Citation

Shahhosseini, Akram; Bourova-Flin, Ekaterina; Derakhshan, Samira; Aminishakib, Pouyan; and Goudarzi, Afsaneh (2023) "High levels of Histone H3 K27 acetylation and tri-methylation are associated with shorter survival in Oral Squamous Cell Carcinoma patients," BioMedicine: Vol. 13 : Iss. 1 , Article 4.
DOI: 10.37796/2211-8039.1391